Cell cycle analysis, morphology quantification, and signaling pathway imaging all depend on accurate intensity measurements. Cytely removes the instrument-dependent variability that makes inter-experiment comparison unreliable.
Use Cases
DNA content quantification (DAPI, Hoechst) requires consistent intensity calibration across experiments. Cytely normalizes nuclear fluorescence intensity to enable reliable G1/S/G2 phase classification even when data were acquired on different microscope configurations.
Cell size, elongation, and cytoskeletal organization measurements are sensitive to imaging system parameters. Cytely's flatfield correction removes illumination-induced spatial artifacts that cause false morphology gradients across the field of view.
Nuclear translocation assays and co-localization measurements depend on channel-specific intensity ratios. Cytely normalizes each fluorescence channel independently — preserving ratio relationships while correcting for inter-instrument gain differences.
Workflow
Collect fluorescence images in your standard acquisition protocol. No changes to your imaging workflow are required.
Apply flatfield correction, background subtraction, and normalization using your saved instrument protocol.
Standardized images export to CellProfiler, MATLAB, or Python. Your existing analysis pipeline runs on data that is now comparable across experiments.
Our application scientists have experience with common cell biology assay types and can advise on protocol configuration.