High-content screening assay quality depends on consistent per-well fluorescence intensity across the entire plate and across all imaging systems used in your campaign. Cytely delivers reproducible Z' factors regardless of which platform the assay runs on.
Use Cases
Primary and counter-screens run across multiple imaging systems over weeks or months. Without standardization, a low Z' factor may reflect imaging variability rather than assay biology. Cytely normalizes each plate to a consistent intensity baseline — isolating biological signal from systematic imaging noise.
EC50 calculations from fluorescence-based viability or reporter assays require monotonic intensity–response relationships. Cytely removes edge effects, non-uniform illumination, and inter-plate intensity drift that distort dose-response curves at the margins of the quantification range.
Morphological profiling (Cell Painting-style) depends on standardized intensity in 5-8 fluorescence channels simultaneously. Cytely applies per-channel correction independently, preserving inter-channel ratios while correcting systematic biases in each detector.
Technical Note
Contact us to discuss your screening infrastructure and integration requirements.