Confocal z-stacks, neurite tracing, and synaptic puncta quantification all require consistent intensity calibration across imaging sessions and microscope configurations. Cytely enables reproducible neuronal morphology and connectivity measurements.
Use Cases
Dendrite and axon length measurements depend on accurate background subtraction to separate neurite signal from non-neuronal autofluorescence. Cytely's per-channel background correction improves neurite detection sensitivity without requiring manual threshold adjustment per image.
Pre- and postsynaptic marker puncta counts (PSD95, synapsin, bassoon) are highly sensitive to intensity thresholds. Cytely normalizes puncta channel intensity to a consistent scale — enabling puncta detection thresholds that generalize across experiments and imaging configurations.
3D fluorescence imaging accumulates illumination artifacts across z-planes. Cytely applies per-slice flatfield correction and z-dependent intensity drift correction — enabling consistent 3D segmentation of neuronal structures throughout the stack depth.
Talk to an application scientist about your specific neuroscience assay and imaging setup.