H&E and IHC tissue section analysis requires consistent stain intensity calibration across scanning platforms and staining batches. Cytely standardizes brightfield and fluorescence tissue images before any quantification runs.
Use Cases
Hematoxylin and eosin staining intensity varies with reagent lot, staining time, and scanner configuration. Cytely normalizes stain density distributions across sections, enabling consistent nuclear and cytoplasmic segmentation thresholds across batches and sites.
Immunohistochemistry DAB intensity is scanner-dependent and batch-sensitive. Cytely's IHC normalization module brings DAB and hematoxylin channel intensities to a common reference frame — enabling reproducible H-score and Allred score calculations across tissue microarrays.
Multi-section and multi-site tumor microenvironment studies require consistent immune cell marker quantification across scanning sessions. Cytely enables direct comparison of CD3, CD8, and PD-L1 expression data collected at different pathology centers.
Contact an application scientist to discuss your IHC or digital pathology standardization needs.